ELYN Water Program

Baseline (BIO), Prevention (DN), Surface Layer / Sediment (DGA).

Pond pH, dissolved oxygen, nitrogen, organic matter, and algae are all interconnected - you cannot treat one in isolation and expect stability. The ELYN Water Program uses three products working together because each targets a different part of this system.

Improved water and sediment quality lowers environmental stress on shrimp - the primary trigger for disease outbreaks. Stressed animals show suppressed immune function and higher susceptibility to viral and bacterial pathogens. This program addresses root causes in the pond environment rather than treating symptoms.

Water column · Baseline

ELYN BIO

Organic matter digestion & microbial baseline

Multi-strain microbial water purifier. Bacillus decomposes proteins, carbohydrates, and lipids; Lactobacillus produces bacteriocins and organic acids that suppress Vibrio; Pseudomonas removes nitrate/nitrite and reduces phosphorus; yeast supports microbial diversity. Reducing the organic load is the most effective biological route to H2S prevention - less anaerobic substrate means less sulfide formed at the pond bottom.

  • Total viable count: ≥ 6 × 10¹² CFU/kg across 6 strains, ≥ 1 × 10¹² CFU/kg per strain
  • Bacillus sp. (2 species, spore-forming) ≥ 2 × 10¹² CFU/kg - multi-enzyme decomposition; denitrification
  • Lactobacillus sp. (2 species) ≥ 2 × 10¹² CFU/kg - bacteriocins, organic acids; suppress Vibrio
  • Pseudomonas sp. ≥ 1 × 10¹² CFU/kg - nitrate/nitrite removal, phosphorus reduction
  • Yeast ≥ 1 × 10¹² CFU/kg - decomposes dissolved lipids, supports microbial diversity
  • Freeze-dried powder, zeolite carrier - 2-year shelf life at room temperature
  • Powder formulation: strains blended post-drying to prevent inter-strain competition
  • Packaging: 5 kg aluminum bags
  • Application: 0.5 to 1 kg/ha weekly, continuous from pond preparation. Increase 2 to 3 times at 60 to 150 PL/m². Double the rate before stocking.
Sediment interface · Nitrogen & H₂S

ELYN DN

Nitrogen cycle & H₂S control

Concentrated nitrifiers and denitrifiers targeting ammonia, nitrite, and hydrogen sulfide (H2S) - the leading non-viral cause of shrimp mortality. Nitrosomonas oxidises ammonia to nitrite, Nitrobacter converts nitrite to far less toxic nitrate, and Bacillus performs heterotrophic denitrification on residual organic load. BIO and DN are never applied in the same week: BIO for two consecutive weeks, then DN in the third. There is no required spacing in days between them.

  • Total viable count: ≥ 6 × 10¹² CFU/kg across 6 strains, ≥ 1 × 10¹² CFU/kg per strain
  • Bacillus sp. (2 species) ≥ 2 × 10¹² CFU/kg - multi-enzyme decomposition; denitrification
  • Lactobacillus sp. (2 species) ≥ 2 × 10¹² CFU/kg - lactobacillin; decomposes dissolved organic matter
  • Nitrosomonas sp. ≥ 1 × 10¹² CFU/kg - nitrification step 1 (NH3 → NO2−)
  • Nitrobacter sp. ≥ 1 × 10¹² CFU/kg - nitrification step 2 (NO2− → NO3−)
  • Freeze-dried powder, zeolite carrier - 2-year shelf life at room temperature
  • Effective across pH and salinity ranges
  • Packaging: 5 kg aluminum bags
  • Application: 0.5 to 1 kg/ha, week 3 of the BIO/BIO/DN cycle. Never the same week as BIO. Same density multiplier as BIO.
Surface & sediment · Algae & minerals

ELYN DGA

Phosphorus capture & algae regulation

Mineral-based - not a probiotic. Calcium, magnesium, and aluminum compounds bind free phosphorus into stable apatite forms that algae cannot use. As the powder sinks it captures phosphorus through the water column, then buffers acidic sediment and reduces H2S toxicity at the bottom. Supplies Ca2+ and Mg2+ essential for shrimp moulting and shell hardening.

  • 77.4% algae removal in 10 minutes in laboratory tests; over 95% total reduction
  • pH buffering against day/night swings caused by photosynthesis cycles
  • Sediment mineralisation alongside bloom control
  • Mineral composition: natural Ca, Mg, Al-based compounds (100-150 mesh)
  • Safety: harmless at 10× recommended dose (200 ppm); non-toxic to daphnia at 50 ppm
  • Packaging: 20 kg bag
  • Application: 5 to 20 ppm, or 60 kg/ha per application
  • Frequency: 2 to 3 times per crop, on trigger rather than on a schedule
  • Triggers: algae bloom or water colour change, pH instability, heavy rain, mineral depletion before moulting
  • Effective duration: up to 1 month

Application timing & adjustment

Timing. ELYN BIO weekly from pond preparation through harvest, never skipped. ELYN DN in week 3 of the three-week cycle, never in the same week as BIO. Increase the amount of BIO and DN with stocking density and feed load, not the interval. ELYN DGA on trigger rather than on a schedule: algae bloom or water colour change, unstable day and night pH, after heavy rain, or mineral depletion before moulting. Two to three applications per crop is the expected total, not a plan.

Adjustment. These are starting rates. Actual dosing is set on the farm and must account for stocking density, pond history and carrying capacity, feed load through the cycle, and any other additives or treatments already in use. Microbial products control pollution only within the range of their biological activity; where load exceeds that range, more product will not compensate.

Pond lifecycle programme

Phase 01
Preparation

BIO · DGA
Establish beneficial microbial community before stocking. Apply BIO to inoculate the water column. Use DGA if algae are present or minerals depleted.
Phase 02
Early Culture

BIO
Maintain BIO weekly to sustain microbial dominance - low organic load phase. Establish competitive exclusion before waste accumulates.
Phase 03
Mid-Cycle Stress

BIO · DN · DGA
Feed load peaks. DN enters the three-week cycle. Increase BIO and DN 2 to 3 times at intensive density. DGA if algae blooms or pH instability.
Phase 04
Risk Management

BIO · DN · DGA
High-density, high-stress phase. Increase DN if ammonia or H2S detected. DGA to control algae before blooms crash. Full programme intensity.

Frequency & dose summary

Product Frequency Dose Timing Key rule
ELYN BIO Weeks 1-2 of 3 0.5-1 kg/ha From prep. Double rate pre-stocking The foundation product, never skipped. Increase the amount with stocking density and feed load, not the interval.
ELYN DN Week 3 of 3 0.5-1 kg/ha Never same week as BIO Introduce when organic load builds. Increase if ammonia or H2S detected.
ELYN DGA 2-3× per crop 5-20 ppm (60 kg/ha) On trigger: algae bloom, pH instability, heavy rain, mineral depletion Reactive and proactive. Apply to knock down blooms - do not wait for a crash.

Stocking density multiplier

Stocking density Weekly BIO or DN
Up to 60 PL/m2 0.5-1 kg/ha
60-150 PL/m2 1-3 kg/ha

Rates assume 60 PL/m2 or below for whiteleg shrimp, or 10 to 30 PL/m2 for black tiger. At intensive and hyper-intensive densities, increase the weekly dose of BIO and DN by 2 to 3 times.

Fish, hatchery and RAS rates

System ELYN BIO or ELYN DN ELYN DGA
Tilapia, all ponds: nursery, fry, grow-out, broodstock, breeding 1 ppm (1 g/m3) once weekly, twice if needed. Alternate BIO and DN where possible, or use either continuously 5-20 ppm per application, or 1-2 ppm weekly
General fish ponds, per 1,000 tonnes of water 1 ppm per week each 5-20 kg per application, or 1-2 kg per week
Hatchery, indoor facilities and RAS 5-10 ppm per week Not applied. Algae-control products are not used in enclosed systems

Monitoring, application & warning signs

Monitoring. Measure pH at 06:00 and 18:00 daily. Daily variation greater than 0.2 signals algal or sediment issues requiring attention. Monitor water colour changes.

Application. Apply products evenly across the pond during daytime in clear weather. Dissolve powder in pond water before broadcasting. Reseal bags and store in a dry, shaded place below 25°C.

Warning signs. Sudden water colour change, foam on surface, strong odour from sediment, or shrimp swimming at the surface all indicate urgent intervention with DN and/or DGA.

Why this works as a system

ELYN BIO handles organic decomposition and microbial baseline - the load that drives oxygen demand and nitrogen stress. ELYN DN targets ammonia, nitrite, and hydrogen sulfide at the sediment-water interface, where H2S is lethal at trace levels easily reached on pond bottoms. ELYN DGA captures phosphorus to starve algae, buffers bottom pH, and supplies minerals - closing the loop when blooms and nutrient imbalance threaten the system.

Surface, water column, and sediment interact constantly. Three products working together - not symptoms, but the root causes of pond instability.

20+ Years

Proven on Korean shrimp farms

≥ 6×1012

CFU/kg total viable count, both BIO and DN

77.4%

DGA algae removal in 10 minutes in laboratory tests; over 95% total

2 years

Shelf life at room temperature (freeze-dried, zeolite carrier)

10×

Safe at 10× recommended dose (no fish toxicity)

Why ELYN

  • DNA-verified strains. Isolated from high-performing Korean ponds over 20+ years. Every strain DNA-verified by an independent Korean genetics laboratory and batch-tested to ≥ 109 CFU/g.
  • Freeze-dried stability. Zeolite carrier ensures a 2-year shelf life. Freeze-drying prevents inter-strain competition - all strains activate simultaneously on contact with water.
  • Spore-forming Bacillus. Heat-resistant endospores survive transport, storage, and harsh pond conditions - guaranteed viable cells at every application.

Observed in commercial conditions

Findings recorded where the programme was applied on working farms. These are observational records, not controlled trials: ponds were not randomised, farms continued their own routines alongside the programme, and the movements described are associations rather than demonstrated product effects.

5.0 → 0.5

Total ammonia, mg/L. A vannamei pond was taken on at total ammonia 5.0 mg/L, nitrite 6.0 mg/L and 33.8 °C. Over the following six weeks, under continuing feed load and peak-season heat, total ammonia fell to 0.5 mg/L.

+63%

Harvest against the same pond's baseline. Stocked 40 percent above the farm's own norm, the pond returned 63 percent more biomass at a larger grade than its previous two cycles. Feed conversion 1.15 against a 1.25 baseline, survival level with baseline, no mortality event across 103 days.

1.2 mg/L

The lowest ammonia peak of three ponds. Three ponds on one farm shared water source, feed brand, aeration and routine. The pond on the ELYN programme was stocked 20 to 35 percent denser, matched the better comparison pond on yield per square metre, and peaked at 1.2 mg/L where both others reached the test kit ceiling of 2.0.

+35 g

Tilapia harvest weight against a competing probiotic. Early morning dissolved oxygen rose from 2.5 to 3.5 mg/L up to 4 to 6 mg/L, microalgae fell by 30 to 50 percent with a shift toward diatoms, and grow-out ponds ran up to 2.5 months without significant water exchange.

Observed results from commercial deployment. Results vary by species and environmental conditions.

Need help adapting the program to your farm?

We can structure a farm-specific rollout with clear execution rules for each product in the program.

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